the interaction between isatin-β-thiosemicarbazone (ibt) and calf thymus dna (ct-dna) was investigated in physiological buffer (ph 7.4) using neutral red (nr) dye as a spectral probe by uv–vis absorption and fluorescence spectroscopy, as well as viscosity measurements. the ibt is stabilized by intercalation in the dna ( k [ibt –dna] =1.03×105 m−1), and displaces the nr dye from the nr–dna comple...

The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV–Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by intercalation in the DNA ( K [IBT –DNA] =1.03×105 M−1), and displaces the NR dye from the NR–DNA comple...

The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV–Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by intercalation in the DNA ( K [IBT –DNA] =1.03×105 M−1), and displaces the NR dye from the NR–DNA comple...

We characterized the acid–base properties, electrochemistry, and electrogenerated chemiluminescence (ECL) of neutral red in acetonitrile (MeCN). To determine the acid–base properties, the basic form (NR) of neutral red was prepared and titrated with anhydrous HClO4, yielding pKa = 6.5 in MeCN. NR showed three irreversible electrochemical oxidations and a one-electron reversible reduction. Howev...

1.0 STANDARD OPERATING PROCEDURE (SOP) FOR THE BALB/C 3T3 NEUTRAL RED UPTAKE CYTOTOXICITY TEST A TEST FOR BASAL CYTOTOXICITY....................... 3 1.1 Background and Introduction.................................................................................................. 3 1.2 Rationale.........................................................................................................

AIM Despite its therapeutic value almost nothing is known about potential adverse health effects of Olea europea L. We therefore investigated the in vitro toxicity and genotoxicity of leaf extracts of this plant. MATERIAL AND METHODS Extracts from olive tree leaves were obtained from four different regions in Tunisia. We investigated the in vitro toxicity, genotoxicity and antigenotoxicity of...

In this study, the adsorption of neutral red (NR) on the spent ground coffee (SGC) was examined. The effect of various parameters such as pH and adsorbent dose on the removal of NR was investigated. The removal ratios of NR (25 and 40 mg/L) on SGC after 2 h were 95.6 and 90.2%, respectively. The removal ratio of NR is increased by increasing the pH value. Equilibrium data fitted very well in a ...

A novel fluorescence sensor of NR-β-CD@AuNPs was prepared for the trace detection of nitrite in quantities as low as 4.25 × 10-3 μg∙mL-1 in an aqueous medium. The fluorescence was due to the host-guest inclusion complexes between neutral red (NR) molecules and gold nanoparticles (AuNPs), which were modified by per-6-mercapto-beta-cyclodextrins (SH-β-CDs) as both a reducing agent and a stabilize...